Species-specific PCR method for identification of Streptococcus downei
نویسندگان
چکیده
منابع مشابه
Identification of Streptococcus sanguinis with a PCR-generated species-specific DNA probe.
The objective of the present study was to design a PCR-generated DNA probe and determine the specificity of the probe for the identification of clinical isolates of Streptococcus sanguinis. To do this, we examined over 200 arbitrarily primed PCR (AP-PCR) amplicon patterns obtained with DNA from clinical isolates of S. sanguinis. A 1.6-kb DNA amplicon that was common to all AP-PCR profiles was e...
متن کاملMultiplex-PCR method for species identification of coagulase-positive staphylococci.
In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease (nuc) genes of staphylococca...
متن کاملA comparison between CHROMagar, PCR-RFLP and PCR-FSP for identification of Candida species
Background and Purpose: The epidemiological alteration in the distribution of Candida species, as well as the significantly increasing trend of either intrinsic or acquired resistance of some of these fungi highlights the need for a reliable method for the identification of the species. Polymerase chain reaction (PCR) is one of the methods facilitating the quick and precise identification of Ca...
متن کاملGroup-specific Multiplex Pcr Identification of Canine Faecal Lactobacillus Species
A set of forty-nine Lactobacillus isolates recovered from the faecal swabs of different dog breeds viz. Pomeranian (12), Labrador (15) and mongrel/indigenous dogs (22) were subjected to Lactobacillus group-specific multiplex PCR corresponding to the 16S-23S rRNA intergenic spacer region (ISR) and its flanking 23S rRNA gene. Twenty-three (46.9%) isolates were found to be positive for Group IV; s...
متن کاملIdentification of Mycobacteria species in cutaneous lesions of Sarcoidosis by PCR-Restriction Ragment Length Polymorphism (PCR-RFLP) method
Background: Sarcoidosis is a granulomatous multisystem disease of unknown etiology. It has recently been tired to detect Mycobacteria genome in biopsy specimens of patients with sarcoidosis by Polymorphism chain reaction method. Objective: To detect and identify Mycobacteria species in cutaneous lesions of the patients with sarcoidosis by PCR-RFLP. Patients and methods: 20 patients with clinica...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2004
ISSN: 0266-8254,1365-2673
DOI: 10.1111/j.1472-765x.2003.01451.x